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pentr4 flag w210 2 entry vector entry vector gift from eric campeau paul kaufman addgene 17423  (Addgene inc)


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    Addgene inc pentr4 flag w210 2 entry vector entry vector gift from eric campeau paul kaufman addgene 17423
    Pentr4 Flag W210 2 Entry Vector Entry Vector Gift From Eric Campeau Paul Kaufman Addgene 17423, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/addgene+entry+vectors/pENTR4-FLAG+(w210-2)+(Plasmid+%2317423)/bio_rxiv__2025__08__26__672361-57-16-27
    Average 93 stars, based on 36 article reviews
    pentr4 flag w210 2 entry vector entry vector gift from eric campeau paul kaufman addgene 17423 - by Bioz Stars, 2026-09
    93/100 stars

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    Luciferase:

    Article Title: Rapid and Efficient Synthetic Assembly of Multiplex Luciferase Reporter Plasmids for the Simultaneous Monitoring of Up to Six Cellular Signaling Pathways.
    Article Snippet: .. Materials Omega destination-CMV:ELuc vector, diluted to 75 ng ml−1 (Addgene #124528) Addgene entry vectors encoding the NF-κβ-responsive RedF luciferase transcriptional unit in alphaA(5×NF-κβ _RE::RedF, Addgene #124530), the SMAD-responsive FLuc luciferase transcriptional unit in alphaB (7×SMAD_RE::FLuc, Addgene #124531), the p53-responsive NLuc luciferase transcriptional unit in alphaD (2×P53_RE::NLuc, Addgene #124533), the MAPK/JNK-responsive GrRenilla transcriptional unit in alphaE (6×AP-1_RE::GrRenilla, Addgene #124534), and the FoxO-responsive Renilla luciferase transcriptional unit in alphaC (3×DBE_RE::Renilla, Addgene #1245435) Restriction enzymes: BsmBI (New England Biolabs, cat. no. R0580), ScaI-HF (New England Biolabs, cat. no. R3122), XhoI (New England Biolabs, cat. no. R0146) T4 DNA ligase and 10× ligase buffer (Promega, cat. no. M1801). .. Home-made chemocompetent E. coli DH10B-T1R cells (ThermoFisher Scientific, cat. no., 12331013; see recipe) 2× LB medium, no antibiotic (see recipe).



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